Moreover, microarray assay in combination with CRNG interference and overexpression showed that the differential genes such as ANPEP, KITLG, STAT5A, FOXP3, miR-451, IL-2, IL-10, IL-6, and TNF-α are mainly involved in viral and pathogens infection and the immune-inflammatory responses in PK-15 cells. Fluorescence in situ hybridization (FISH) showed that CRNG mainly distributes in cytoplasm. Hence, we identified the novel gene as long non-coding RNA (lncRNA) and named it cyadox-related novel gene (CRNG). Here, rapid amplification of cDNA ends (RACE) and bioinformatics analysis showed that the novel gene is 953 bp without protein-coding ability and locates in chromosome 11.
Our previous study has showed that a novel gene is differentially expressed in the liver of cyadox-fed piglets, but its sequence and function are unknown. 2Ministry of Agriculture Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China.1National Reference Laboratory of Veterinary Drug Residues and Ministry of Agriculture Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, China.Qirong Lu 1,2, Li Li 1, Aixin Huang 1, Luqing Cui 2, Yinfeng Zhang 2, Qianying Liu 1, Xu Wang 1,2, Yulian Wang 2, Zhenli Liu 2, Zonghui Yuan 1,2* and Menghong Dai 1,2*
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